![]() Apoptosis was assessed using the TUNEL technique and TNFα mRNA by in situ RT-PCR. Anti-inflammatory activity was assessed in vitro by inhibition of TNFa and nitrite production from RAW 264.7 cells exposed to LPS (50 ng/ml) and in vivo using the indomethacin-induced gastritis model. ![]() Alkaloids and flavanols were determined using reversed-phase HPLC scavenging of 1,1-diphenyl-2-picrilhydrazyl (DPPH), hydroxyl radicals, and lipid peroxidation by spectrophotometry and TNFα production by ELISA. The purpose of this study was to characterize the antioxidative and antiinflammatory properties of cat's claw, Uncaria tomentosa (UT) and Uncaria guianensis (UG). ![]() Cat's claw is an herbal medicine from the Amazon that is used widely to treat inflammatory disorders. ![]()
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